Technical Data
C2617-02M3
CENTG3 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 3, AGAP-3, CRAM-associated GTPase, CRAG, Centaurin-gamma-3, Cnt-g3, MR1-interacting Protein, MRIP-1, AGAP3)
Description:
CENTG3, or M-RIP, is a 116kD protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Immunohistochemistry (Paraffin): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-NPSAELHRSPSLL, from human AGAP3, at C-terminal (NP_114152.3).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human AGAP3. Species sequence homology: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Surks HK, Richards CT, Mendelsohn ME. Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. J Biol Chem. 2003 Dec 19;278(51):51484-93. Epub 2003 Sep 23.