Technical Data
C3050-01
CETP (Cholesteryl Ester Transfer Protein)
Description:
It has been proposed that high density lipoproteins (HDLs) function jointly with lecithin:cholesterol acyltransferase and CETP to facilitate cholesterol transport from tissues to the liver. This mechanism, referred to as reverse cholesterol transport, is physiologically important because it maintains systemic cholesterol levels. CETP is responsible for neutral lipid transfer activity in plasma in numerous species. Since CETP is able to specifically accelerate the exchange of lipid components between pro- and anti-atherogenic lipoprotein fractions, it may be a key determinant of the global atherogenicity of the plasma lipoprotein profile and arises as a possible target in atherosclerosis prevention. CETP has an important role in reverse cholesterol transport and shaping and affecting the composition of plasma lipoproteins. In general, elevated levels of CETP have been associated with increased risk of coronary heart disease.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:400. Detects a doublet at ~70kD from human serum representing CETP.
Immunoprecipitation: Immunoprecipitates CETP activity from serum samples using Protein G.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15D2Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide corresponding to aa131-142, CDSGRVRTDAPD from the C-terminal of human CETP protein. Cellular Localization: Secreted
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human cholesteryl ester transfer protein (CETP). Species Crossreactivity: rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Largost, L., Biochemica and Biophysica Acta. 1215: 209-236 (1994). 2. Thomas, A.P., et al., Hybridoma 15(5): 359-364 (1996). 3. Medical Science Research 21: 911-912 (1993).