Technical Data
C3490
CHAPS ((3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate))
5g
25g
100g
25g
100g
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Technical Data |
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C3490 |
CHAPS ((3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate)) |
5g 25g 100g |
| Biochemicals | Storage: -20°CShipping: RT |
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Non-denaturing zwitterionic detergent with cholic acid and sulfobetaine moieties, used to dissolve biological membranes, useful for protein extraction methods due to its ease of removal by dialysis or gel filtration. Low background UV absorption for protein detection. Designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes. Melting Point: 270-272°C Solubility: Methanol (Sparingly), Water Atmosphere: Inert Gas Stability: Hygroscopic and Moisture Sensitive Method for Determing Identity: H NMR Spectroscopic Analysis TLC Conditions: SiO2; Methanol: NH4OH; 19: 1; Visualized with AMCS; Rf=0.3 CAS Number: 75621-03-3 Molecular Formula: C32H58N2O7S Molecular Weight: 614.9 |
Source: Synthetic Purity: ~90% Form: Supplied as a white to off white powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Hjelmeland, L. H., Proc. Natl. Acad. Sci. USA, 77: 6368 (1980) 2. Good, N. E., et al., Biochemistry 5:467 (1966)
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