		Technical Data
C4250-41	Chlamydia sp., Multi-epitope Cocktail (FITC)

Description:
Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: paraformaldhyde-fixed tissue sections. Detects chlamydia species in specimens after fixation with methanol/acetone (1:1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG3	9j118, 9j119, 9j120	Affinity Purified

Size		Storage	Shipping	Source	Host
1.5ml		-20°C	Blue Ice		Mouse

Concentration:

Not determined

Immunogen:

Chlamydia antigen.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a lyophillized powder in 0.09% sodium azide and a protein stablizer. Lbeled with fluorescein isothiocyanate (FITC). Reconstitute in 1.5ml of sterile dH2O.

Specificity:

Recognizes Chlamydia sp. Reacts with the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated antigen(s) directly in samples. Reactive with C. trachomatis, C. pneumoniae, C. psittaci.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Näher H, Petzoldt D, Sethi KK. Evaluation of non-radioactive in situ hybridisation method to detect Chlamydia trachomatis in cell culture. In: Genitourin Med 64:162-164 (1988)