Technical Data
C4250-51F
Chlamydia LPS
Description:
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella Minnesota or Neisseria gonorrhoeae.

Applications:
Suitable for use in Immunofluorescence Microscopy, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Immunofluorescence Microscopy: 1:10 with PBS, pH 7.4.
Immunohistochemistry: Paraformaldehyde-fixed tissue sections. Detects chlamydia species in samples after fixation with methanol/acetone (1:1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG35F153Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Chlamydia antigen
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes a genus-specific epitope of the Chlamydia lipopolysaccharide antigen and identifies 15 serotypes of C. trachomatis as well as C. psittaci and C. pneumoniae with a strong fluorescence of the intracellular inclusions, the pinhead-sized extracellular elementary bodies and the free cell-associated Chlamydia lipopolysaccharide antigens (amorphous foci).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Näher, H., Petzoldt, D., Sethi, K.K. Evaluation of non-radioactive in situ hybridisation method to detect Chlamydia trachomatis in cell culture. In: Genitourin Med. 64: 162-164 (1988).