Technical Data
C4250-53C
Chlamydia trachomatis
Description:
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus. Chlamydia has a genome size of approximately 500-1000kb and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
-Kenneth Todar, University of Wisconsin-Madison

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry (paraffin). Other applications not tested

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG3,k11C208Affinity Purified
SizeStorageShippingSourceHost
1mg4°C Do Not FreezeBlue IceMouse
Concentration:
~4mg/ml
Immunogen:
Chlamydia trachomatis.
Purity:
Purified by Protein A affinity chromatography. ~90% (SDS-PAGE).
Form
Supplied as a liquid in 10mM sodium phosphate, 150mM sodium chloride, pH7.4, 0.05% sodium azide.
Specificity:
Recognizes a major surface protein of Chlamydia trachomatis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hori, S., et al.,(1995), “Histological Differentiation Between Chlamydial and Bacterial Epididymitis:
Nondestructive and Proliferative Versus Destructive and Abscess Forming-Immunohistochemical and
Clinicopathological Findings”, Hum. Pathol., 26: 402-407.