Technical Data
C4363-10C
Chloride Intracellular Channel 1 (CLIC 1, CLIC1, Chloride Channel ABP, G6, hRNCC, Nuclear Chloride Ion Channel 27, NCC27, p64CLCP, Regulatory Nuclear Chloride Ion Channel Protein, RNCC Protein)
Description:
Chloride intracellular channel protein 1 (CLIC1) is a part of the CLIC family of proteins. This family of proteins reconstitute in phospholipid vesicles and have been shown to have chloride channel action. CLIC1 may rotate between membrane-bound and soluble forms since it has been seen as a membrane protein and in the cytoplasm. When there is a lack of detergent, CLIC1 becomes membrane-bound and can act as an anion-selective channel. Recently, it has been shown that CLIC1 is required in angiogenesis and has a part in controlling integrin cell surface expression.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.25ug/ml detects CLIC1 on 10ug of human liver tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k11C234Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Full length recombinant protein corresponding to human CLIC1. Material obtained from the National Cancer Institutes Clinical Proteomic Technologies for Cancer (CPTC) program.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human CLIC1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.