		Technical Data
C4363-10C	Chloride Intracellular Channel 1 (CLIC 1, CLIC1, Chloride Channel ABP, G6, hRNCC, Nuclear Chloride Ion Channel 27, NCC27, p64CLCP, Regulatory Nuclear Chloride Ion Channel Protein, RNCC Protein)

Description:
Chloride intracellular channel protein 1 (CLIC1) is a part of the CLIC family of proteins. This family of proteins reconstitute in phospholipid vesicles and have been shown to have chloride channel action. CLIC1 may rotate between membrane-bound and soluble forms since it has been seen as a membrane protein and in the cytoplasm. When there is a lack of detergent, CLIC1 becomes membrane-bound and can act as an anion-selective channel. Recently, it has been shown that CLIC1 is required in angiogenesis and has a part in controlling integrin cell surface expression.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.25ug/ml detects CLIC1 on 10ug of human liver tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	11C234	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Full length recombinant protein corresponding to human CLIC1.Material obtained from the National Cancer Institute’s Clinical Proteomic Technologies for Cancer (CPTC) program.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human CLIC1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.