Technical Data
C5057-06B
Choline Acetyltransferase (CHAT)
Description:
Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein(640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250 -1:500
Immunohistochemistry: 1:100 -1:200
Immunocytochemistry: 1:100 -1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanChicken
Concentration:
Not determined
Immunogen:
Peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P_066264, NCBI) and mouse (Q03059,NCBI) sequences.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 10mM; isotonic 0.9%, with 0.02% sodium azide.
Specificity:
Recognizes human CHAT. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.