Technical Data
C5067-70L
Chondroitin Sulfate Proteoglycan (BSA & Azide Free) (NG2, CSPG)
Description:
The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors1. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias2. Studies indicate that NG2 is involved in modulating cellular response to growth factors 3-5 and cell motility 6-7; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries8. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration 8-9. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis10. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors11.

Applications:
Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Other applications not tested.

Recommended Dilution:
Immunofluorescence Microscopy: Cells in culture
Immunoprecipitation: 1ug/10e6 cells
FACS analysis: 1-2ug/10e6 cells
Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a2Q550Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS pH 7.6, 0.25M sodium chloride. No preservative added. Glycerol free. Also available with azide. See C5067-70.
Specificity:
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stallcup WB, et al. J Neurocytol 31(6-7):423-35, 2002.
2. Stallcup WB, et al. J Cell Sci 114:2315-2325, 2001.
3. Grako KA and Stallcup WB. Exp Cell Res 221(1):231-40, 1995.
4. Grako KAJ, et al. Cell Sci 112(6):905-15, 1999.
5. Goretzki L, et al. J Biol Chem 274(24):16831-7, 1999.
6. Burg MA, et al. Exp Cell Res 235(1):254-64, 1997.
7. Fang X, et al. Mol Biol Cell (10):3373-87, 1999.
8. Chen ZJ, et al. J Neurocytol 31(6-7):481-95, 2002.
9. Jones LL, et al. J Neurosci 22(7):2792-2803, 2002.
10. Chekenya M, et al. FASEB J 16:586-588, 2002.
11. Chekenya M, et al. J Neurocytol (6-7):507-21, 2002.
General References:
1. Bumoi, T.F. et al. (1984) J. Biol. Chem. 258: 1273312741. 2. Morgan Jr., A.C. et al. (1981) Hybridoma 1: 2735. 3. Schrappe, M. et al. (1992) Cancer Res. 52: 38383844. 4. Schrappe, M. et al. (1991). Cancer Res. 51: 498693