Technical Data
C5069-66A
Chorionic Gonadotropin, Human, beta (hCGb)
Description:
Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a- and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Recommeded Pair:
Capture: C5069-54 or C5069-60
Detection: C5069-66A

Affinity Constant:
2.1x10e11L/mole

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19.F.55Highly Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human Chorionic Gonadotropin, beta
Purity:
Chromatography purified
Form
Supplied as a liquid in 0.9% sodium chloride, 0.1% sodium azide.
Specificity:
Recognizes hCG beta and HCG. Crossreactivity: HCGa <1.0%; hTSH <0.1%; hLH and hFSH <0.5%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Faulk, W.P. & Taylor, G.M., An Immunocolloid, Method for the Electron Microscope. Immunochemistry. 8: 1081-1083 (1971). 2. Chandler, J., Gurmin, T., and Robinson, N. 2000. The Place of Gold in Rapid Tests. IVD Technology 6(2): 37-49. 3. Hayat, M.A., ed. 1989. Colloidal Gold: Principles, Methods, and Applications, vol. 1. San Diego: Academic Press. 4. Handley, D.A., 1989. Methods for Synthesis of Colloidal Gold. page 22 in Hayat, M.A., ed. Colloidal Gold: Principles, Methods, and Applications. San Diego: Academic Press. 5. Frens, G., Controlled Nucleation for the Regulation of Particle Size in Monodisperse Gold Solutions. Nature Physical Science. 20: 241 (1973).