		Technical Data
C5069-66A	Chorionic Gonadotropin, Human, beta (hCGb)

Description:
Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a- and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Affinity Constant:
2.1x10e11L/mole

Recommeded Pair:
Capture: C5069-54 or C5069-60
Detection: C5069-66A

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	9.F.55	Highly Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Purified human Chorionic Gonadotropin, beta

Purity:

Chromatography purified

Form

Supplied as a liquid in 0.9% sodium chloride, pH 7.4, 0.02% sodium azide.

Specificity:

Recognizes hCG beta and HCG. Crossreactivity: HCGa <1.0%; hTSH <0.1%; hLH and hFSH <0.5%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Faulk, W.P. & Taylor, G.M., An Immunocolloid, Method for the Electron Microscope. Immunochemistry. 8: 1081-1083 (1971). 2. Chandler, J., Gurmin, T., and Robinson, N. 2000. The Place of Gold in Rapid Tests. IVD Technology 6(2): 37-49. 3. Hayat, M.A., ed. 1989. Colloidal Gold: Principles, Methods, and Applications, vol. 1. San Diego: Academic Press. 4. Handley, D.A., 1989. Methods for Synthesis of Colloidal Gold. page 22 in Hayat, M.A., ed. Colloidal Gold: Principles, Methods, and Applications. San Diego: Academic Press. 5. Frens, G., Controlled Nucleation for the Regulation of Particle Size in Monodisperse Gold Solutions. Nature Physical Science. 20: 241 (1973).