Technical Data
C5069-68
Beta-Chorionic Gonadotropin, Free, Human, BioAssay ELISA Kit
1Kit
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Technical Data |
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C5069-68 |
Beta-Chorionic Gonadotropin, Free, Human, BioAssay ELISA Kit |
1Kit |
| Kits and Assays | Storage: 4°CShipping: Blue Ice |
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Intended Use: For the quantitative determination of free beta subunit of human chorionic gonadotropin (free Beta-hCG) concentration in human serum. Standard Size: 96 wells Range: 0-50ng/ml Sensitivity: 2.5ng/ml Crossreactivity: This kit has been validated for use with human serum samples only. Principle of the Test: The free beta-hCG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the free beta-hCG. Mouse monoclonal anti-free-beta-hCG antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-whole hCG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the free beta-hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 30 minute incubations at 37°C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of beta-hCG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. Kit Components: C5069-68A: Microtiter Plate, 1x96wells (in strips). Coated with murine monoclonal anti-free beta-hCG antibody C5069-68B: Calibrator 0ng/ml, 1x1vial. Lyophilized. C5069-68C: Calibrator 2.5ng/ml, 1x1vial. Lyophilized. C5069-68D: Calibrator 5ng/ml, 1x1vial. Lyophilized. C5069-68E: Calibrator 10ng/ml, 1x1vial. Lyophilized. C5069-68F: Calibrator 25ng/ml, 1x1vial. Lyophilized. C5069-68G: Calibrator 50ng/ml, 1x1vial. Lyophilized. C5069-68H: Sample Diluent, 1x13ml C5069-68J: Pab (HRP), 1x18ml C5069-68K: TMB Solution, 1x11ml C5069-68L: Stop Solution, 1x11ml Storage and Stability: Store kit components at 4°C. Unopened kit components are stable for 6 months at 4°C. Reconstituted standards are stable for up to 30 days when stored sealed at 4°C. |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Engall, E., Enzyme immunoassay ELISA and EMIT. In: Van Vunakis, H. and Langone, J.J. (eds.), Methods in Enzymology, Academic Press, New York, 1980; 70:419-439. 2. Densem, J., and Wald, N.J., The stability of blood samples for the measurement of the free beta subunit of chorionic gonadotrophin. Prenat. Diagn., 1995; 15: 94-95. 3. Hay, D.L., Placental histology and the production of human choriogonadotrophin and its subunits in pregnancy. Br. J Obstet. Gynaecol., 1988; 95:1268-1275. 4. Ozturk, M., Bellet, D., Manil, L., et. al., Physiological studies of human chorionic gonadotropin (hCG), alpha hCG, and beta hCG as measured by specific monoclonal immunoradiometric assays. Endocrinology, 1987; 120:549-558. 5. Ozturk, M., Berkowitz, R., Goldstein, D., Bellet, D., Wands, J. R., Differential production of human chorionic gonadotropin and free subunits in gestational trophoblastic disease. Am. J. Obstet. Gynecol.,1988; 158:193-198. 6. Cole, L.A., Hartle, R.J., Laferla, J.J., et al., Detection of the free beta subunit of human chorionic gonadotropin (hCG) in cultures of normal and malignant trophoblast cells, pregnancy sera, and sera of patients with choriocarcinoma. Endocrinology, 1983; 113:1176-1178. 7. Gaspard, U.J., Reuter, A.M., Devėlle, J-L, et al., Serum concentration of human chorionic gonadotropin and its alpha and beta subunits. Clin. Endocrinol. (OXF), 1980; 13:319-329.
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