Technical Data
C5069-96J
Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Comments: Excellent for staining of formalin/paraffin with no special pretreatment.

Applications:
Immunohistology (Formalin/paraffin): (Use Ab 1:200 for 30 min at RT) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Positive Control: Pancreas

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG5G82Supernatant
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant protein encoding human chromogranin A.
Purity:
Culture supernatant
Form
Supplied as a liquid with 0.09% sodium azide.
Specificity:
Recognizes Chromogranin A. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Spieker-Polet H, et al. Proc Natl Acad Sci. 1995 Sep 26; 92(20): 9348-52. 2. Liguori MJ, et al. Hybridoma. 2001 Jun; 20(3):189-98. 3. G.Cano1, F. Milanezi2,et al.,1Garcia da Orta Hospital, Almada, Portugal,2 Inst. Molec. Pathology and Immunology of Porto University, Portugal,3 Medical Faculty of Porto university, Portugal Diagn Cytopathol, 2003 Oct; 29(4): 207 -11. 4. L.K. Diaz* and N.Sneige *Department of Pathology,Northwestern University, Chicago,+ Department of Pathology, University of Texas, Huston, Adv Anat Pathol,2005; 12(1), 10-19. 5. Z. Huang1, W. Zhu2, G. Szekeres3, H. Xia1 1Spring Bioscience Corp, Fremont,CA, 2 Epitomics Inc, Burlingame,CA, , 4Histopathology Ltd, Hungary, Appl Immunohistochem Mol Morphol. 2005; 13 (1): 91-95.