Technical Data
C5071-50C
ChT1 (PE)
Description:
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function.3 This antibody can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers.4

Applications:
• Identification and enumeration of ChT1+ cells by flow cytometry 4
• Immunoprecipitation1,4
• In vitro inhibition of T cell differentiation4

Recommended Dilution:
Flow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K101Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceChickenMouse
Concentration:
~0.1mg/ml
Immunogen:
Chicken thymocytes
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
Specificity:
Recognizes the ChT1 antigen (Mr 63/104kD) on chicken thymocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen, C.L., H.T.C. Chanh, and M.D. Cooper. 1984. Chicken thymocyte-specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization. Eur. J. Immunol. 14:385.
2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.
3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.
4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.