Technical Data
C5073-06J
Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)
1 Vial
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 199aa of human Ciliary Neurotrophic Factor expressed in E.coli is a single, non-glycosylated polypeptide chain.

Molecular Weight:
~22.7kD

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 5x10 IU/mg.

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~22.7kD
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Relation of weight maintenance and dietary restraint to peroxisome proliferator-activated receptor gamma2, glucocorticoid receptor, and ciliary neurotrophic factor polymorphisms. Am J Clin Nutr 2005 Oct;82(4):740-6. 2. Ciliary neurotrophic factor-immunoreactivity in olfactory sensory neurons. Neuroscience 2005;134(4):1179-94. 3. Ciliary neurotrophic factor promotes muller glia differentiation from the postnatal retinal progenitor pool. Dev Neurosci 2004;26(5-6):359-70. 4. Dedifferentiation of adult human myoblasts induced by ciliary neurotrophic factor in vitro. Mol Biol Cell 2005 Jul;16(7):3140-51/ 5. Rapid protein kinase C-dependent reduction of rat skeletal muscle voltage-gated sodium channels by ciliary neurotrophic factor. J Physiol 2005 Jun 15;565(Pt 3):827-41. 6. E xpression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats. Neurosci Lett 2005 Apr 4;377(3):170-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.