|Biochemicals||Storage: -20°CShipping: RT|
Ciprofloxacin Hydrochloride is a high activity fluoroquinolone antibiotic effective against a broad spectrum of gram positive and negative bacteria such as pseudomonas, salmonella, streptococcus and staphylococcus. Effective against mycoplasma (12 days).
Ciprofloxacin differs from other quinolones in that it has a fluorine atom at the 6-position, a piperazine moiety at the 7-position, and a cyclopropyl ring at the 1-position.
The bactericidal action of ciprofloxacin results from interference with the enzyme DNA gyrase which is needed for the synthesis of bacterial DNA.
Ciprofloxacin has been shown to be active against most strains of the following organisms:
Gram-Positive Bacteria: Enterococcus faecalis (Many strains are only moderately susceptible); Staphylococcus haemolyticus; Staphylococcus hominis; Staphylococcus saprophyticus; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus pneumoniae; Streptococcus pyogenes.
Gram-Negative Bacteria: Acinetobacter calcoaceticus subs. anitratus; Acinetobacter calcoaceticus subs. Iwoffi; Aeromonas caviae; Aeromonas hydrophila; Brucella melitensis; Campylobacter coli; Campylobacter jejuni; Citrobacter diversus; Citrobacter freundii; Edwardsiella tarda; Enterobacter aerogenes; Enterobacter cloacae; Escherichia coli; Haemophilus ducreyi; Haemophilus influenzae; Haemophilus parainfluenzae; Klebsiella oxytoca; Klebsiella pneumoniae; Legionella pneumophila; Moraxella (Branhamella) catarrhalis; Morganella morganii; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella multocida; Proteus mirabilis; Proteus vulgaris; Providencia rettgeri; Providencia stuartii; Pseudomonas aeruginosa; Salmonella enteritidis; Salmonella typhi; Serratia marcescens; Shigella flexneri; Shigella sonnei; Vibrio cholerae; Vibrio parahaemolyticus; Vibrio vulnificus; Yersinia enterocolitica.
Other Organisms: Chlamydia trachomatis (only moderately susceptible); Mycobacterium tuberculosis (only moderately susceptible).
C5074: Ciprofloxacin Hydrochloride
C5075: Ciprofloxacin Hydrochloride 10mg/ml
Form: Supplied as a lyophilized powder.
Appearance: Faintly yellowish to light yellow crystalline powder
Acetic acid: 0.14g/dL
In water and 0.1M NaOH: Solutions are stable to hydrolysis for 16 hrs. at 90°C.
In 0.1M HCl: Displays slight decarboxylation.
Turbidity can be cleared by heat to 30-50°C
pH: 3.0-4.5 (The pH for 0.2% infusion solutions is 3.5-4.6).
Heavy Metals: 0.002%
Effective at low concentrations (10ug/ml) in water and buffered media.
Storage and Stability:
Store powder at -20°C. Reconstitute with suitable solvent. Aliquot and store at -20°C. Solutions should be protected from light. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
US Biological application references: 1. Sweeney, L.G. et al. (2005) Int J Pharm. 305:180-185. 2. Mani, N. et al., (2006) Antimicrobial Agents and Chemotherapy 50:1228-1237. 3. Callegan, M.C. et al., (2006) Curr Eye Res. 31:693-702. 4. Hoti, S. et al., (2006) Molecular Therapy (2006) 14:768-778. 5. Ely, L et al., (2007) Eur J Pharm Biopharm. 65:346-353. 6. Hoti, s. et al., (2010) Cancer Gene Therapy 17; 585-597.