Technical Data
c-Jun, phosphorylated (Ser63) Control Peptide
Molecular Biology Storage: RTShipping: RT
c-Jun is a member of the Jun Family composed of c-Jun, JunB and JunD, and is a component of
the transcription factor AP-1. AP-1 is composed dimers of Fos, Jun and ATF family members, and binds and activates transcription at TRE/AP-1 elements (1). Extracellular signals including growth factors, chemokines and extracellualar stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues
and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and the context of the cell (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as promising therapeutic target
for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).

Corresponding antibody, C5815-10, detects endogenous levels of c-Jun only when phosphorylated at Ser63. Does not recognize the phosphorylated forms of JunD or JunB. Species Crossreactivity: Mouse and rat.

Suitable for use as a blocking peptide to evaluate the specificity of antibody reactivity in immunohistochemistry and Western blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Purified
Concentration: As reported
Form: Supplied as a lyophilized powder.

Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Jochum, W. et al. (2001) Oncogene 20, 2401–12. 2. Davis, R.J. (2000) Cell 103, 239–52. 3. Hilberg, F. et al. (1993) Nature 365, 179–81. 4. Raivich, G. et al. (2004) Neuron 43, 57–67. 5. Behrens, A. et al. (2002) EMBO J 21, 1782–90. 6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690–700. 7. Leppδ, S. and Bohmann, D. (1999) Oncogene 18, 6158–62. 8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131–6. 9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111–3. 10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109–20. 11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177–81. 12. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111–3. 13. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63,

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.