Technical Data
C5815-10D
c-Jun Sandwich ELISA, BioAssay™, Antibody Pair
1Kit
Kits and Assays Storage: 4°C/-20°CShipping: Blue Ice
Total c-Jun Sandwich ELISA Antibody Pair is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and a HRP-Conjugated Secondary Antibody (1000X stock) is supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-Conjugated Secondary Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.

A. Kit Components:
Total c-Jun Capture Antibody (100X), 1x400ul
Total c-Jun Detection Antibody (100X), 1x4000ul
Anti-mouse IgG (HRP) (1000X), 1x40ul

B. Reagents Required But Not Supplied:
1. Coating Buffer: 1X PBS, (20X PBS) 3.2 mM Na 2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, pH 7.4
2. Wash Buffer: 1X PBS/0.05% Tween-20, (20X PBST)
3. Blocking Buffer: 1X PBS/0.05% Tween-20, 1% BSA
4. 1X Cell Lysis Buffer: (10X Cell Lysis Buffer) 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM ethylene diamine tetraacetate (EDTA), 1 mM ethylene glycol-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA), 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM
Na3VO4, 1 g/ml leupeptin.
5. TMB Substrate: (TMB Substrate)
6. STOP Solution: (STOP Solution)
NOTE: Reagents should be made fresh daily.


C. Storage and Stability
Store Total c-Jun capture and detection antibodies at 4°C. Store Anti-mouse IgG (HRP) at -20°C.
Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Binetruy, B. et al. (1991) Nature 351, 122–127.
(2) Smeal, T. et al. (1991) Nature 354, 494–496.
(3) Derijard, B. et al. (1994) Cell 76, 1025–1037.
(4) Kyriakis, J.M. et al. (1994) Nature 369, 156–160

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.