Technical Data
C5815-10D
c-Jun Sandwich ELISA, BioAssay™, Antibody Pair
1Kit
Kits and Assays Storage: 4°C/-20°CShipping: Blue Ice
c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements. Extracellular signals including growth factors, chemokines, and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK. Knock-out studies in mice have shown that c-Jun is essential for embryogenesis, and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration, liver regeneration, and T cell development. AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context. Other target genes regulate survival, as well as hypoxia and angiogenesis. Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis.

c-Jun Sandwich ELISA, BioAssay™, Antibody Pair is offered as an alternative to our c-Jun, Total, Sandwich ELISA Kit (Qualitative) #C5815-10H. Capture and Detection antibodies (100X stocks) and HRP-Conjugated Secondary Antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-Conjugated Secondary Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.

Kit Components:
C5815-10D1: Total c-Jun Capture Antibody (100X), 1x400ul, pink cap color
C5815-10D2: Total c-Jun Detection Antibody (100X), 1x4000ul, blue cap color
C5815-10D3: Anti-mouse IgG (HRP) (1000X), 1x40ul, yellow cap color

Storage and Stability:
Store C5815-10D1 and C5815-10D2 at 4°C. Store C5815-10D3 at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.