Technical Data
C5828-03
Clara Cell Protein (CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein, CCSP)
Description:
Uteroglobin belongs to the family of secretoglobins and is a secreted protein product of nonciliated bronchiolar Clara cells. There is convincing data suggesting it has phospholipase A2 inhibitory activity, as well as a number of other immunomodulatory features, including inhibition of interferon gamma signalling and Th1 vs. Th2 lymphocyte regulation. It was proposed as a potential peripheral marker of respiratory epithelial injury and bronchial dysfunction. Clara Cell Protein 16 concentrations have been determined in both serum and bronchoalveolar lavage fluid in numerous studies since 1994. In serum, its increase is associated with age, asbestos, nitrogen chloride and ozone exposure, sarcoidosis and high PEEP ventilation. Decreased serum CC16 levels are found after pulmonary resection, in silica-exposed workers, smokers and in asthma. Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome). In pleural effusions, the CC16 concentration appears to be associated with its diffusion from the lung as evidenced by high CC16 levels in cardiac pleural congestion.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:2000 dilution detected CCSP in paraffin-embedded mouse lung sections. Paraffin-embedded adult mouse lung sections were stained with C5828-03. Proteins were visualized using a biotinylated goat anti-rabbit secondary antibody and an ABC detection system.
Western Blot: Reported to detect CCSP in mouse lung nuclear extracts.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa45-68 of mouse Clara Cell Secretory Protein, conjugated to KLH.
Purity:
Serum
Form
Supplied as a liquid, 0.05% sodium azide, 30% glycerol
Specificity:
Recognizes mouse Clara Cell Secretory Protein, Mr 10kD. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological Application References: 1. ter Horst, S.A.J., et al., BMC Pulm. Med. 6: 8 (2006). General References: 1. Stripp, B.R., et al., Am. J. Physiol. (Lung Cell. Mol. Physiol.) 271: L656-664 (1996). 2. Harrod, K.S., et al., Am. J. Physiol. (Lung Cell. Mol. Physiol.) 275: L924-L930 (1998). 3. Chang, A., et al., Ann. N.Y. Acad. Sci. 923: 181-192 (2000). 4. Shijubo, N., et al., Curr. Pharm. Des. 9: 1139-1149 (2003).