Suitable for use in Immunohistochemistry (frozen tissue) and Immunoblot (Western).
Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT.
Immunoblot: 1:100-1:500 (ECL).
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Coated vesicles (clathrin) of bovine brain.|
|Supplied as a lyophilized powder. Reconstitute to 1ml final volume.|
|Species Crossreactivity: Human, bovine, porcine, rat, amphibia. Reactivities on Cultured HeLa, SV-40, RVF-SMC Cell Lines (tested so far). Specifically Detected organs and tissues (eg brain, mammary gland, ovaries). This clone represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. refs. 2 and 3). Polypeptide Reacting Mr 180,000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Bruder G and Wiedenmann B: Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues. Exp. Cell Res. 164, 449-462 (1986)|
2. Hirst J and Robinson MS: Clathrin and adaptors. Biochim. Biophys. Acta 1404, 173-193 (1998)
3. Qualmann B, Kessels MM and Kelly RB: Molecular links between endocytosis and the actin cytoskeleton. J. Cell Biol. 150, F111-F116 (2000)