Technical Data
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.

Cellular Localization:
Cytoplasmic vesicle; cytoplasmic vesicle membrane; cytoplasmic side. Cell membrane; coated pit; cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. PTM: The N-terminus is blocked. Belongs to the clathrin heavy chain family.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in m
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
This antibody has been shown to be specific for clathrin. Species Crossreactivity: Rat, other species have not yet tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kirchhausen T, et al. Proc. Natl. Acad. Sci. U.S.A. 84:8805-8809(1987). ; 2. Ter Haar E, et al. Cell 95:563-573(1998). ; 3. Chi A, et al. J. Proteome Res. 5:3135-3144(2006). ; 4. Beausoleil S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). ; 5. Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003). ; 6. Dodge G.R, et al. Genomics 11:174-178(1991). ; 7. Nomura N, et al. DNA Res. 1:27-35(1994).