Technical Data
Claudin 5 (CLDN5)
Tight junctions (TJ) are specialized regions of cell-cell adhesion in epithelial and endothelial cell sheets. Claudins are the major components tight junction strands. In the brain and lung, claudin-5 was exclusively concentrated at cell-cell borders in endothelial cells of blood vessels, but not at those of epithelial cells. In contrast, claudin-5 was restricted to endothelial cells of arteries, but not veins and capillaries in the kidney.1 It is also reported that Claudins-2,-3,-4 and -5 showed heterogenic expression and subcellular localization in the rat liver, pancreas and gut.2 Mouse anti-Claudin-5 stained 100% (20/20) of colon cancer tissues with their matched adjacent and remote mucosa. The staining patterns in both the cancer tissues or the matched mucosa are cytoplasmic and membranous. In breast carcinoma, 62% (33/53) of the tissues stained positive, but the staining pattern was mainly cytoplasmic.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemisty (FFPE): Requires Heat Induced Epitope Retrieval (HIER) using EDTA pH 8.0. Tested in human samples only. Enzyme digestion is not required.
Immunohistochemistry: Frozen sections
Western Blot: 1-3ug/ml. Tested in mouse and rat samples only.
Optimal dilutions to be determined by the researcher.

Positive Control:
Colon Tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceMouseMouse
Synthetic peptide corresponding to mouse claudin-5 protein.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Recognizes human Claudin 5 at ~22-24kD. Expected Staining Pattern: Cytoplasmic/Membrane. Species Crossreactivity: mouse and rat (Western Blot only).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morita K, et al. J Cell Biol. 147:185-194, 1999. 2. Rahner C, et al. Gastroenterology 120(2):411-422, 2001.