CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.
Suitable for use in Western Blot and Chromatin Immunoprecipitation. Other applications not tested.
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not Determined |
|Two peptides derived from mouse CLOCK, conjugated to albumin.Cellular Localization: Cytoplasmic and nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus|
|Supplied as a liquid, 0.05% sodium azide.|
|Recognizes mouse CLOCK. Species Crossreactivity: human |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Hogenesch, JB., et al. The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors. Proc. Natl. Acad. Sci USA. 95(10):5474-9, 1998. 2. Dunlap, Jay. An End in the Beginning. Science. 280: 1548-49, 1998. 3. Jin, X., et al. A Molecular Mechanism Regulating Rhythmic Output from the Suprachiasmatic Circadian Clock. Cell. 96: 57-68, 1999. 4. Shearman, L.P. and D.R. Weaver. Photic induction of Period gene expression is reduced in Clock mutant mice. Neuroreport. 10(3): 613-618, 1999.|