		Technical Data
C5843-02B	CLOCK, ID (Circadian Locomoter Output Cycles Protein Kaput, hCLOCK, Class E Basic Helix-loop-helix Protein 8, BHLHE8, KIAA0334)

Description:
CLOCK belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within this protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region of human CLOCK (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CLOCK.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Sookoian,S., Am. J. Clin. Nutr. 87(6), 1606-1615 (2008). 2.Nakashima,A., Mol. Cell. Biol. 28(12), 4080-4092 (2008). 3.Marino,J.L., Epidemiology 19(3), 477-484 (2008).