Technical Data
C5870-06
Clusterin, beta, Rat (SGP-2, Sulfated Glycoprotein-2, DAG, Dimeric Acidic Glycoprotein, TRPM-2, Testosterone Repressed Prostate Message-2)
Description:
Clusterin, which is also known as Sulfated Glycoprotein-2 (SGP-2), Dimeric Acidic Glycoprotein (DAG), and Testosterone Repressed Prostate Message-2 (TRPM-2), is a disulfide-linked heterodimeric sulfated glycoprotein expressed in a variety of tissues. Although its function is unknown, clusterin binds to cells, membranes and hydrophobic proteins. It also inhibits complement activation. Induced or elevated levels of clusterin have been associated with programmed cell death caused by hormonal stimuli or traumatic insult.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml. Detects rat clusterin (70kD) in rat serum under non-reducing conditions.
Immunohistochemistry: 10ug/ml. Detects rat clusterin in rat brain sections fixed with 4% paraformaldehyde/2% acetic acid in PBS.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-04D IgG, H&L (AP) Pab Gt x Mo
I1904-06D IgG, H&L, X-Adsorbed (AP) Pab Gt x Mo
I1904-06J IgG, H&L, X-Adsorbed (AP) Pab Gt x Mo
I1904-09X IgG, X-adsorbed (AP) Pab Gt x Mo
I1904-25D IgG, H&L (AP) Pab Gt x Mo
I1904-67AA IgG, H&L (AP) Pab Gt x Mo
I1904-67AB IgG H&L, X-adsorbed (AP) Pab Gt x Mo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG0.T.20Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat clusterin expressed in yeast.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in Tris-glycine, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes rat beta-clusterin at 70kD under non-reducing conditions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collard, M.W. and Griswold, M.D., Biochemistry 26: 3297-3303 (1987). 2. Bandyk, M.G., et al., J. Urol. 143: 407-413 (1990). 3. Wong, P., et al., J. Biol. Chem. 268: 5021-5031 (1993).