Technical Data
C6200X
CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase) (BSA & Azide Free)
Description:
CNPase (2,3-Cyclic Nucleotide 3-Phosphodiesterase) exists in two forms: 46kD (CNP1) and 48kD (CNP2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtually absent in other cell types. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA1 familial breast cancer gene.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin/paraffin): 2-4ug/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Brain

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.224Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase). Cellular Localization: Cell membrane
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide, cat# C6200.
Specificity:
Recognizes human proteins of 46kD (CNP1) and 48kD (CNP2), identified as two forms of CNPase. Species crossreactivity: monkey, bovine, sheep, goat, porcine, feline, canine, rabbit, rat and mouse. Does not react with guinea pig or chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sprinkle TJ, et. al. Brain Research, 426:349-357, 1987. 2. Sprinkle TJ, et. al. CRC Critical Reviews in Neurobiology, 4:235-301, 1989.