Technical Data
C6400
Cytotoxic Necrotizing Factor 1 (CNF1)
Description:
CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis
and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter
exhibits the catalytic activity of the toxin.

Applications:
Suitable for use in Immunoassays, Western Blot, Native dot blot analyses, and for inhibition of biological activity. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9G169Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceE. coliMouse
Concentration:
As reported
Immunogen:
Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. Recognizes an epitope between aa704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meysick, K et al; Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect Immun 2001, 69: 2066 2. McNichol, B et al; Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein. Infect Immun 2007, 75: 5095