Technical Data
Collaborator of ARF (CARF)
CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
As reported
E. coli-derived recombinant human CARF (aa380-580) GenBank Accession # NM_017632
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, 0.02% sodium azide or sterile 40-50% glycerol.
Recognizes human CARF.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.