Technical Data
C7510-10W
Collagen Type I
Description:
The extracellular matrix is the material found in the extracellular environment of all tissues and organs. It consists of basement membranes and interstitial stroma. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens, each with unique features suited for its function and location. The collagens are proteins composed of three subunit polypeptides that can vary in length, which interact to form a triple helix. The molecular basis of the triple helix is provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptides generated are capable of assembly into fibrillar or other types of supramolecular assemblies which are deposited in the extracellular matrix. The number of distinct collagen types presently recognized is more than fourteen.

Applications:
Suitable for use in ELISA, Dot Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:2000; The epitope recognized may be sensitive to routine formalin fixation and paraffin embedding. In testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen.
Optimal dilutions to be determined by the researcher.

Recommended Tissue:
Human, porcine tongue and human, porcine tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J1052Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Full length, purified bovine native protein. Cellular Localization: Extracellular matrix.
Purity:
Ascites
Form
Supplied as a liquid, 15mM sodium azide.
Specificity:
Recognizes the native (helical) form of bovine Collagen type I at ~95kD. Does not react with the thermally-denatured molecule. No crossreactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI. Species Crossreactivity: human, porcine, rabbit, deer, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sanes, J.R., et al., J. Cell Biol. 111: 1685 (1990). 2. Werkmeister, J.A. and Ramshaw, J. A., Acta Derm. Venereol. (Stockh.) 69: 399 (1989). 3. Gay, S. and Fine, J.D., Methods Enzymol. 145: 148 (1987). 4. Mayne, R., Clin. Biochem. 21: 111 (1988).