Technical Data
C7510-13
Collagen Type I
Description:
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600 in length and 15 in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Applications:
Suitable for use in RIA, Immunofluorescence, ELISA and Immunohistochemistry. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence (frozen): 1:80
ELISA: 1:200
Immunohistochemistry (paraffin): 1:500
Optimal dilutions to be determined by the researcher.

Titer:
RIA: 1:200

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Purified Collagen Type I from mouse skin.
Purity:
Purified by column chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes mouse Collagen Type I: 100%. Crossreactivity: Mouse Collagen Type III: < 1% Mouse Collagen Type II, IV: < 0.1% Human, chicken, rat collagen Type I: < 0.1%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.