Technical Data
Collagen IV
The collagen family is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral selfassociation in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function, such as bone strength and platelet activation.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:1000 on formalin fixed, paraffin embedded and enzyme treated human tongue sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanMouse
Not Determined
Human collagen type IV
Supplied as a liquid, 15mM sodium azide.
Recognizes native, undenatured human Collagen IV . Using immunohistochemical staining of basal laminae, recognizes an epitope located on the alpha1 and/or alpha2 chains of human Collagen IV. Does not crossreact with human collagen types I, II, III, V, VI, or VII. Does not react with human vitronectin, fibronectin or chondroitin sulfate types A, B and C. Species Crossreactivity: primate
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sanes JR et al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990). 2. Gay S & Fine JD Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry. Methods Enzymol 145:148-67 (1987). 3. Sage H et al. Structural studies on human type IV collagen. J Biol Chem 254:9893-900 (1979).