Technical Data
C7510-52K
Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 2 Cha
Description:
Human plasma proteins do not interfere with collagen binding. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina.

Applications:
Suitable for use in ELISA, Immunofluorescence, and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
immunohistochemistry (Frozen): 1:10-1:50
immunohistochemistry (Paraffin): 1:10-1:50
ELISA: 1:1,500-1:6,000
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul4°C/-20°C (No Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Collagen type IV from human placenta.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from mannitol, dextran and salts. Reconstitute with 500ul sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes human collagen IV. Reactivities with collagen IV and related proteins: human collagen IV 100% , human types I, III and V < 10%, human collagen II <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.