Technical Data
Collagen Type V
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 5-10ug/ml; reducing conditions; detects ~160kD band
Immunohistochemistry: 1-10ug/ml using PLP-fixed, paraffin-embedded tissue. PLP fixation is required; antigen retrieval is HIER; not recommended for traditional formalin fixation; acetone fixation can also be used.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug4C (-20C Glycerol)Blue IceHumanMouse
Human Collagen Type V
Supplied as a liquid in 0.1M PBS, pH 7.0, 2% BSA (Protease free), before the addition of glycerol to 40%.
Recognizes human type V collagen (CL(V)) at ~160kD. Species Crossreactivity: rabbit and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mayne, R. et al. (1980). Artery 7: 262280. 2. Niyibizi, C. et al. (1984). J. Biol. Chem. 259: 14170- 14174.