Technical Data
Collagen Type VI
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.

Suitable for use in ELISA, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:10-1:20
Immunohistochemistry (frozen): 1:10-1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ug-20CBlue IceHumanGoat
Human type VI collagen.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Reacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10% crossreactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.