Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
ELISA: 1:2000-1:4000. 1ug of Collagen VI was used in a standard sandwich ELISA with Goat anti-Rabbit IgG [H&L] (HRP conjugated) and (ABTS (2,2'-azino-bis- [3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
Western Blot: 1:250-1:2500
Immunohistochemistry (Frozen tissue sections): 1:25-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|50ug||4°C (-20°C Glycerol)||Blue Ice||Rabbit|
|Collagen Type VI from human and bovine placenta|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.|
|Reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
US Biological application references: 1. Hoben, G.M. (2009) Stem Cells Dev. 18:283-292. 2. Natoli, R.M. (2009) Tissue Engineering Part A. 15:3119-3128. 3. Hoben, G.M. and Athanasiou, K.A. (2007) Biotechnology and Bioengineering 100:587-598. 4. Ofek, G. et al., (2008) PLoS ONE, 3:e2795.|