Technical Data
C7850-13G
Complement C3a, C3a des-Arg (Neo-Epitope)
Description:
The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. Monoclonal antibodies detecting neo-epitopes have been used for direct quantification of activation at different steps in the complement cascade. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b.
C3a is a mediator of local inflammatory processes. C3a does induce smooth muscle contraction, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:50 used with a reduced sample treatment and SDS-PAGE. Detected a band at ~8kD.
Optimal dilutions to be determined by the researcher.

Positive Control:
Activated human serum or purified human C3adesArg protein

Negative Control:
Any irrelevant protein

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A529Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human C3a des-Arg
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes a neo-epitope on human C3a (des-Arg) that is formed upon cleavage of C3 into C3a and C3b. Recognizes both C3a and C3a-desArg, with at least 5 times higher preference for C3a-desArg.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.