Technical Data
C7853-01A
Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)
Description:
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL Recognizes a band of ~25kD in mouse liver.

ELISA: 1:100,000 using 50-100ng CX26 control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A 19 amino acid peptide sequence near the C-terminal, 2nd cytoplasmic domain of the mouse Cx26 (KLH coupled)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse connexin 26. Species sequence homology: rat (100%), monkey (94%), human (89%), sheep, bovine and dog (8%), rat CX30 (63%). No significant hmology with other connexins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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3. Zhang, J-T and Nicholson BJ (1994) J Membrane Biol. 139, 15-29;
4. Goliger J & Paul D (1994) Devel. Dynamics 200, 1-13
5. Zhang JT and Nicholson BJ (1989) J cell Biol. 109, 3391-3401; Nicholson B et al (1987) nature 329, 732-734; Lee Sw et al (1992) J Cell Biol. 118, 1213