		Technical Data
C7853-06A	Connexin 31, CT, C241 (Gap Junction beta-3 Protein, Connexin-31, Cx31, GJB3)

Description:
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa234-264 from the C-terminal region of human GJB3.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human GJB3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mhatre, A.N., et al., Clin. Genet. 63(2):154-159 (2003). 2. Diestel, S., et al., Biochem. Biophys. Res. Commun. 296(3):721-728 (2002). 3. Di, W.L., et al., Hum. Mol. Genet. 11(17):2005-2014 (2002). 4. Gottfried, I., et al., Hum. Mol. Genet. 11(11):1311-1316 (2002). 5. Wenzel, K., et al., Biochem. Biophys. Res. Commun. 248(3):910-915 (1998).