Technical Data
C7853-61
Connexin 30
Description:
The connexin gene family codes for the protein subunits of gap junction channels, which mediate the direct diffusion of ions and metabolites between the cytoplasm of adjacent cells. The gap junction channels have unique properties depending on the type of connexins constituting the channel.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry (Frozen Sections): 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouseRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide derived from the unique C-terminus of the mouse Connexin 30 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Connexin 30. Crossreactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Species Crossreactivity (confirmed by Western Blot using brain cell lysates): human, feline and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Saez, J.C., et al., Advances in Second Messenger and Phosphorylation Research (1993). 2. Bennet, M.V.L., et al., Neuron 6: 305-320 (1990). 3. Beyer, E., et al., J. Cell. Biol. 105: 2621-2629 (1987). 4. Beyer, E.C., J. Cell. Biol. 108: 595-605 (1989). 5. Crow, D.S., et al., Mol. Cell. Biol. 10: 1754-1763 (1990). 6. Fishman, G.I., et al., J. Cell. Biol. 111: 589-598 (1990). 7. Kanter, H.L., et al., Circ. Res. 73: 344-350 (1993). 8. Wilgenbus, K.K., et al., Int. J. Cancer 51: 522-529 (1992). 9. Davis, L.M., et al., J. Amer. Coll. Cardiol. 24: 1124-1132 (1994). 10. Dahl, E., et al., J. Biol. Chem. 271: 17,903-17,910 (1996). 11. Nagy, J.I., et al., Neuroscience 78: 533-548 (1997). 12. Rash, J.E., et al., J. Neuroscience 21(6): 1983-2000 (2001). (Also used for freeze-fracture immunogold labeling for double labeling and electron microscopy). 13. Nagy, J.I., et al., Neuroscience 88(2): 447-468 (1998).