Technical Data
C7853-61B
Connexin 31.1 (CX31.1.1, Gap Junction Beta 5 Protein, CXB-5)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 1:100K using 50-100ng CX31.1 control peptide/well.
Optimal dilutions to be determined by the researcher

Control Peptide: C7853-61C Connexin 31.1, Rat Control Peptide (CX31.1.1, Gap Junction Beta 4 Protein, CXB-4)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceRatRabbit
Concentration:
1mg/ml
Immunogen:
A 14 amino acid synthetic peptide C-terminal, cytoplasmic of rat Cx31.1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of 40% glycerol.
Specificity:
Recognizes rat Connexin 31.1 Does not have significant homology with other known Connexins. Species sequence homology: mouse 86%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; White , WT et al (1995) Kidney Intl. 48, 1148-1157; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194
2. Haefliger, JA et al (1992) J. Biol. Chem. 267, 2057
3. Goliger, J et al (1994) Dev. Dynamics 200, 1-13
4. Hennemann, H et al (1992) J. Biol. Chem. 267, 17225-17233