Technical Data
C7855-16
Connexin 36 (CX36)
Description:
The murine Cx36 gene encodes a protein of 321 amino acids most homologous to connexin 35. Based on the presence of an intron within its coding region the Cx36 gene is suggested to form a new delta subclass of murine connexin genes. Cx36 is the first gap junction protein expressed predominantly in neuronal cells of the mammalian central nervous system. It is highly expressed in adult retina and is present in neurons of the inferior olive, the olfactory bulb, the CA3/CA4 hippocampal subfields and several brain-stem nuclei. Cx36 mRNA expression in brain increases gradually during fetal development until day 7 post-partum when its expression begins to decline. Biophysical measurements of gap junction channels formed by transfected and endogenous Cx36 indicate that they posess unique properties well suited for mediating flexible electrical and biochemical interactions between neurons.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1.0ug/ml
Immunohistochemistry: Frozen sections; Reactivity was con rmed with frozen sections from rat brain (stained: olfactory bulb and inferior olive), retina (stained: inner and outer plexiform layers, inner nuclear layer) and spinal cord (stained: gray matter).
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Peptide corresponding to a sequence located in the cytoplasmic loop between the second and third transmembrane domains of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes rat and mouse Cx36 protein (~36kD). Speci city was con rmed by Western Blot with lysates from rat retina and olfactory bulb. A homodimer may be observed. Species sequence homology: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, M. & Gilula, M.B., Cell 84: 381-388 (1996). 2. Saez, J.C., et al., In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al., Neuron 6: 305320 (1990). 4. Kuraoka, A., et al., J. Histochem. and Cytochem. 41: 971980 (1993). 5. Wilgenbus, K.K., et al., Int. J. Cancer 51: 522529 (1992). 6. Condorelli, D.F., et al., Eur. J. Neurosci. 10: 12021208 (1998). 7. Sohl, G., et al., FEBS Lett. 428: 2731 (1998). 8. Srinivas, M., et al., J. Neurosci. 19(22): 98489855 (1999).