Technical Data
Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)
Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46kD protein. Cx46, along with Cx50, is principally expressed in the lens of the eye. Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells. Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function. Mutations of Cx46 result in severe cataracts of the lens.

Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml. Identifies a single band at ~46kD
Immunofluorescence: 3ug/ml
Immunohistochemistry (Frozen): 3ug/ml
Note: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to an internal region of the mouse Connexin 46 protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes mouse Connexin 46 protein. Species Sequence Homology: rat, Chinese hamster and Golden hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. White, T.W., Science 295: 319-320 (2002). 2. Bannerman, P., et al., J. Neurosci. Res. 61: 605-615 (2000). 3. Hopperstad, M.G., et al., Biophys. J. 79: 1954-1966 (2000). 4. Pal, J.D., et al., Am. J. Physiol. Cell Physiol. 279: C596-602 (2000). 5. Mackay, D., et al., Am. J. Hum. Genet. 64: 1357-1364 (1999). 6. Jiang, H., et al., Mol. Vision 9: 579-593 (2003).