		Technical Data
C7901-36L	CORO1A (Coronin-1A, Coronin-like Protein A, Clipin-A, Coronin-like Protein p57, Tryptophan Aspartate-containing Coat Protein, TACO, CORO1)

Description:
Coronin 1 was first isolated from Dictyostelium, but similar proteins have been identified in many species and individual cell types. The coronin-like protein in yeast promotes actin polymerization and also interacts with microtubules. Dictyostelium mutants lacking coronin are impaired in cytokinesis and all actin-mediated processes. Analysis of coronin-GFP fusions and knockout mutants shows that coronin participates in the remodelling of the cortical actin cytoskeleton that is responsible for phagocytosis and macropinocytosis. Likewise, in mammalian neutrophils, a coronin-like protein is also associated with the phagocytic apparatus. Coronin 1 binds actin and is found in noninfected macrophages, localised to the cortical microtubule network; in mycobacteria-infected macrophages, progressively relocalized and retained around the mycobacterial phagosomes.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: >1:16,000
Western Blot: 0.25-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat or Molt-4

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to KRLDRLEETVQAK, from the C-terminus of the protein sequence according to NP_009005.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human CORO1A. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ferrari G, Langen H, Naito M, Pieters J.A coat protein on phagosomes involved in the intracellular survival of mycobacteria.Cell. 1999 May 14;97(4):435-47. PMID: 10338208 2. Honoré B et al., Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci. 2008 Jun 10;6:18. PMID: 18544163.