Technical Data
C7901-83J
Cortactin (CTTN, Amplaxin, EMS1, FLJ34459, Oncogene EMS1, Src Substrate Cortactin)
Description:
Cortactin is a c-src substrate associated with sites of dynamic actin assembly at the leading edge of migrating cells promotes branched actin filament network formation by Arp2/3 complex. Cortactin is an F-actin-binding protein. The amino-terminal half of the protein is comprised of multiple copies of an internal, tandem 37- amino acid repeat while the carboxyl-terminal half contains a distal SH3 domain. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate its importance for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. human cortactin gene is localized on chromosome 11q13.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Cortactin. Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wu, H. et al. The Journal of Cell Biology. 120:1417-1426 (1993).