Technical Data
Cortactin (CTTN, Amplaxin, EMS1, FLJ34459, Oncogene EMS1, Src Substrate Cortactin)
Cortactin is a c-src substrate associated with sites of dynamic actin assembly at the leading edge of migrating cells promotes branched actin filament network formation by Arp2/3 complex. Cortactin is an F-actin-binding protein. The amino-terminal half of the protein is comprised of multiple copies of an internal, tandem 37- amino acid repeat while the carboxyl-terminal half contains a distal SH3 domain. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate its importance for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. human cortactin gene is localized on chromosome 11q13.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic non-phosphopeptide corresponding to human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recognizes human Cortactin. Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wu, H. et al. The Journal of Cell Biology. 120:1417-1426 (1993).