Technical Data
C7901-87
Cortactin, phosphorylated (Tyr486)
Description:
Cortactin is an 80-85kD actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml; The antibody detects an 80kD protein from 30ug EGF/A431 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding to residues surrounding phosphorylated Tyr486 of human cortactin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS with 0.05% Bronidox.
Specificity:
Recognizes human Cortactin phosphorylated on tyrosine 486.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.