		Technical Data
C7901-87	Cortactin, phosphorylated (Tyr486)

Description:
Cortactin is an 80-85kD actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml; The antibody detects an 80kD protein from 30ug EGF/A431 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Peptide corresponding to residues surrounding phosphorylated Tyr486 of human cortactin.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS with 0.05% Bronidox.

Specificity:

Recognizes human Cortactin phosphorylated on tyrosine 486.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.