Technical Data
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
COX-2 has been cloned from a variety of species including human, rat, and sheep and has a molecular weight of 72kDa.1,2,3,4, COX-2 from these species are approximately 90% identical at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%. Expression of COX-2 is regulated by growth factors, tumor promoters, bacterial endotoxin and cytokines, including IL-1 beta.5,6 COX-2 is present in human breast tumors but is not expressed in normal breast tissue.7

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml (1:200)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1Vial4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptide corresponding to aa 567-599 (S567VPDPELIKTVTINASSSRSGLDDINPTVLLKE599) of human COX-2.
Supplied as a liquid in TBS, pH 8.0, containing 50% glycerol and 0.02% sodium azide.
Recognizes human COX-2. Species Crossreactivity: sheep. Does not cross-react with COX-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1 Hla, T., et al. Proc Natl Acad Sci USA: 89 7384-7388 (1992). 2 Kujubu, D.A., et al.,. J Biol Chem 266: 12866-12872 (1991). 3 Yamagata, K., et al. Neuron 11: 371-386 (1993). 4 Zhang, V., et al.,. Biochem Biophys Res Commun 227: 499-506 (1996). 5 Camacho, M., et al.,. J Biol Chem 270: 17279-17286 (1995). 6 Croxtall, J.D., et al., Biochem Biophys Res Commun 220: 491-495 (1996). 7 Parrett, M.L., et al., Int J Oncol 10: 503-507 (1997).