Technical Data
C7904-82V
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
Description:
COX catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of COX by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothosis that the inducible form of COX, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.1,2 COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:300-1:600
Western Blot: 1:200
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1Vial4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to C-terminal aa 570-598 (D570PQPTKTATINASASHSRLDDINPTVLIK598) of mouse/rat COX-2
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 0.5mg/ml BSA, 0.02% sodium azide and 50% glycerol.
Specificity:
Recognizes mouse COX-2. Species Crossreactivity: human, rat, sheep, rabbit, monkey and guinea pig COX-2. Does not cross-react with COX-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.