Technical Data
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
COX catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of COX by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothosis that the inducible form of COX, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.1,2 COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep.

Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:300-1:600
Western Blot: 1:200
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
1Vial-20CBlue IceMouseRabbit
Synthetic peptide corresponding to C-terminal aa570-598 (D570PQPTKTATINASASHSRLDDINPTVLIK598) of mouse/rat COX-2, conjugated to BSA.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from TBS, pH 7.4, 5mg/ml BSA. Reconstitute with 500ul sterile dH2O.
Recognizes mouse COX-2. Species Crossreactivity: human, rat, sheep, rabbit, monkey and guinea pig COX-2. Does not cross-react with COX-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.