		Technical Data
C7904-83DE	COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Description:
Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved in the conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar in structure and function, though they vary in expression. COX-1 is normally expressed in most cell types, whereas COX-2 expression is at low levels unless induced by hormonal stimuli. The apparent molecular weight of COX-2 appears to be around 70kD.
COX-2 is the inducible isoform of cyclooxygenase. COX-2 is upregulated in response to number of mediators, including growth factors, cytokines and endotoxins. COX-2 appears to be expressed in several different types of human cancers, including sporadic coloreactal, breast, pancreatic and cervical cancers. COX-2 immunoreactivity is also present in neurons at postsynaptic sites in rat cerebral cortex. Besides its role in carcinogenesis, COX-2 is also involved in apoptosis, cellular adhesion as well as angiogenesis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 10-20ug/ml. Formalin-fixed, paraffin-embedded tissue sections require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	6A53	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide derived from COX-2.

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes human COX-2 protein at ~70kD. Reactivity has been confirmed by Western Blot analysis with stimulated human endothelial cell lysates.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Charpigny, G., et al., Endocrinology 138(5): 2163-2171 (1997). 2. Ballif, B.A., et al., PNAS 93: 5544-5549 (1996). 3. Chapple, K.S., et al., Amer. J. Pathol. 156(2): 545-553 (2000). 4. Spizzo, G., et al., Br. J. Cancer 88(4): 574-578 (2003). 5. Okami, J., et al., J. Exp. Clin. Cancer Res. 21(4): 569-576 (2002). 6. Ferrandina, G., et al., J. Clin. Oncol. 20(4): 973-981 (2002). 7. Kaufmann, W.E., et al., PNAS 93: 2317-2321 (1996). 8. Tsuji, M. & DuBois, R.N., Cell 83: 493-501 (1995). 9. Tsuji, M., et al., Cell 93: 705-716 (1998).