Technical Data
COX 2 (Cyclooxygenase-2, Cox2, COX-2, PHS II, Prostaglandin G/H Synthase 2, PGH Synthase 2, Prostaglandin H2 Synthase 2, PGHS-2, Prostaglandin-endoperoxide Synthase 2, Ptgs2)
The cyclo-oxygenase (Cox) enzyme catalyses the oxidation of arachidonic acid into prostaglandin H2. The enzyme is encoded by two related genes, Cox-l and Cox-2. The Cox-1 gene is constitutively and ubiquitously expressed, while the Cox-2 gene is only expressed at high levels upon induction by growth factors, cytokines and extracellular stimuli associated with cell activation. Many cell types associated with inflammation, such as monocytes, endothelial cells and fibroblasts, express the Cox2 gene upon induction. Besides there is evidence that it is expressed constitutively, however, the expression is usually low under physiological conditions. It has a role as a major mediator of inflammation and a role for prostanoid signaling in activity-dependent plasticity. It is expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Lung and colon carcinoma

Cellular Localization:
Cytoplasmic and membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceRatRabbit
Not determined
Recombinant protein corresponding to c-terminus of rat COX2.
Supplied as a liquid in 0.05% sodium azide.
Recognizes rat COX2. Species Crossreactivity: mouse and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Susan et al. Biochem J 302, 723, 1994. 2. Yamagata et al. Neurom 2: 371, 1993. 3. Baber et al. Circulation 108: 896,2003.