Technical Data
CREB (cAMP Response Element Binding Protein)
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).

Suitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:250
Chromatin Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:400; Use citrate as an unmasking buffer.
Immunohistochemistry (Frozen): 1:400
Immunofluorescence: 1:1600
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
GST-CREB full length fusion protein
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Recognizes endogenous levels of total human CREB protein at ~43kD. Species Crossreactivity: mouse, rat, monkey, D. melanogaster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lonze, B.E., et al., Neuron 34: 371-385 (2002). 2. Lee, M.M., et al., J. Neurosci. Res. 55: 702-712 (1999). 3. Redmond, L., et al., Neuron 34: 999-1010 (2002). 4. Dash, P.K., et al., Nature 345: 718-721 (1990). 5. Yin, J.C., et al., Cell 79: 49-58 (1994). 6. Guzowski, J.F. and McGaugh, J.L., Proc. Nat. Acad. Sci. USA 94: 2693-2698 (1997). 7. Xing, J., et al., Mol. Cell. Biol. 18: 1946-1955 (1998). 8. Ribar, T.J., et al., J. Neurosci. 20: RC107 (2000). 9. Tan, Y., et al., EMBO J. 15: 4629-4642 (1996).