Technical Data
CREB Regulated Transcription Coactivator 1 (CRTC1, FLJ14027, KIAA0616, MECT1, TORC1, WAMTP1)
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:500. Validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Western Blot: 1:500-1:2000
ELISA: 1:10,000-1:40,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide, KLH-conjugated. Epitope: Amino acids 19-34 of human MECT1 protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Recognizes Human CREB Regulated Transcription Coactivator 1 (CRTC1). Species Crossreactivity: Bovine, Mouse, Rat, Zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.